DP21681653 Production of saplings of rare wild fruit species of Zhetysu Alatau by vegetative reproduction

News and events

Project Implementation Report

As part of the project implementation, the university has created the necessary material and technical conditions:

  • Allocation of a plot for the nursery
    A 72 m² plot has been allocated for the establishment of a nursery. The allocated area has been fenced, and a nursery for wild fruit plants has been established. Mother plants of wild fruit species such as Sievers apple, Niedzwetzky apple, common apricot, Almaty hawthorn, Dzungarian hawthorn, Ili barberry, Ili rosehip, and Meyer’s currant have been planted in this nursery.
  • Establishment of a laboratory
    A plant micropropagation laboratory is operational in the new educational and laboratory building under the Research Institute of Biotechnology and Ecology. The laboratory is equipped with modern equipment and consumables funded by the grant and the university’s non-budgetary resources. The laboratory focuses on obtaining and propagating explants of wild fruit species.
  • Construction of a greenhouse
    A modern greenhouse has been built for cultivating and propagating seedlings obtained through green cutting and micropropagation.
  • Collection of raw materials
    Necessary initial plant materials were collected during field trips to the Almaty and Zhetysu regions.
  • Development of a technological process
    A technological process scheme for green cutting of wild fruit plants was developed. This scheme has been implemented for obtaining green cuttings, resulting in 25 seedlings of each species for propagation in the nursery.
  • Introduction of in vitro technology
    Protocols for in vitro introduction have been developed for each species of wild fruit plants. A clean culture has been obtained, and micropropagation was carried out for species classified as difficult-to-root. Micropropagation technologies for each species were developed, microcuttings were performed, and seedlings were obtained for greenhouse cultivation and nursery planting.
  • Marketing activities
    To promote the obtained products, a website was developed, video materials were created, and brochures, leaflets, and a catalog were published. Methodological guidelines were also prepared. Market research was conducted to identify consumers, and contracts for product sales and future cooperation were signed. The total revenue from product sales amounted to 2,300,000 KZT.

Key Project Deliverables:

  1. Development of methodological guidelines
    Methodological guidelines on “Vegetative Propagation of Wild Fruit Species of Zhetysu Alatau” were prepared and published. This publication is registered with the International Standard Book Number (ISBN): 978-601-216-985-0.
  2. Creation of a catalog
    A catalog was developed and printed, containing systematic information about wild fruit species, their characteristics, and vegetative propagation methods.
  3. Production of informational materials
    Brochures and leaflets were prepared as part of the project to raise awareness about wild fruit plants and promote their conservation among the target audience.
  4. Development of the project website
    An official project website was created, featuring key materials, including the methodological guidelines, catalog, and up-to-date information about the project and its achievements.

 

Scientific internships and professional development

1. Bakhtaulova A., Oksikbayev B., Atabayeva A., Ukusheva T., KanagatovaA. Technology of Malus Sieversii softwood cuttingfor conservation of wild fruit forests on theterritory of Zhongar-Alatau National Park in Kazakhstan PP-APR12-022Abstracts / Journal of Biotechnology 305S (2019) S56–S57https://doi.org/10.1016/j.jbiotec.2019.05.200

2. Bakhtaulova A., Abdildauly, A., Ukusheva, T.K., Atabayeva, A.M. Structure of the radial parenchyma of annual shoots of wild species of Tian-Shan mountainash (Sorbus tianschanica Rupr.) IOP Conference Series: Earth and Environmental Science, 2021, 677(4), 042045 doi:10.1088/1755-1315/677/4/042045

3. Bakhtaulova A., R K Karipbayeva Cytostructure of the radial parenchyma of annual shoots of wild species of Meyer’s currant (Ribes Meyeri Maxim.) AGRITECH-III-2020 IOP Conf. Series: Earth and Environmental Science 548 (2020) 072021 IOP Publishing doi:10.1088/1755-1315/548/7/072021

1. Карашолакова Л. Н., Кушнаренко С. В., Мухитдинова З. Р., Мухитдинов Н. М.Илийская Искра (Lonicera iliensis Pojark.) особенности размножения растений клоном и биологического роста семян // Вестник КазНУ. Серия биологическая. – 2011. – № 6 (52). — С. 120-123. КОКСОН

2. Kushnarenko S.V., Aralbaeva M.M., Karasholakova L.N., Romadanova N.V., Krasavin V.F., Rakhimbaev I.R. Establishing in vitro shoot culture of potato for cryogenic bank development in Kazakhstan // Abstracts of 15th European Congress on Biotechnology, Istanbul, Turkey, September 23-26, 2012. – P. 191. WoSQ1

3. Кушнаренко С. В., Карашолакова Л. Н., Абидкулова к. т. Сохранение ex situ редкого и исчезающего казахстанского вида Lonicera iliensis // Plant biology and biotechnology international conference. Almaty, May 28-30, 2014. P. 262.

4. Kushnarenko S.V., Karasholakova L.N.,Romadanova N.V.Ex situplant conservation biotechnology in Kazakhstan // Abstracts of 17th European Congress on Biotechnology, Lecce, Italy, 15-18 May, 2014. S30.WoSQ2

5.Ромаданова Н. В., Мишустина С. А., Карашолакова Л. Н., Аралбаева М. М., Кабулова Ф. Д. Абидкулова К. Т., Кушнаренко С. В. Введение в культуру in vitro дикорастущих видов Berberis флоры Казахстана и Узбекистана // Вестник КазНУ. Серия биологическая. — Алматы, 2015. –№ 3 (65). — С. 346-354. КОКСОН

6. Romadanova N.V., Mishustina S.A., Karasholakova L.N., Reed B.M., Kushnarenko S.V Creation of an in vitro collection of Berberis spp // International conference on “New approaches in biotechnology and biosciences”. Agra, India, 2016 – — Р. 10.

7. Romadanova N.V., Mishustina S.A., Karasholakova L.N., Reed B.M., Kushnarenko S.V. In vitro collection of cultivars and wild forms of Berberis spp for creation the cryobank // Journal of Biotechnology. – 2016. – V. 231. – P. S39. (Abstracts of European Biotechnology Congress, Riga, Latvia, 05-07 May 2016). WoSQ2

8. Ромаданова Н. В., Мишустина С. А., Карашолакова Л. Н., Аралбаева М. М., Рахимбаев И. Р., Кушнаренко С. В. Создание коллекций in vitro дикорастущих видов Berberis sp. // Бюллетень Государственного Никитского ботанического сада №121. — 2016 г.-С. 69-76.

9. Кушнаренко С. В., Ромаданова Н. В., Карашолакова Л. Н., Мишустина С. А., Аралбаева М. М., Жоламанова С. Ж., Рахимбаев И. Р. Усовершенные протоколы криоконсервации апикальных меристем для создания криобанки генетических ресурсов растений / / материал VII Международной научной- практической конференции, посвященной 30-летию отделения биотехнологии подтвержденного Никитского ботанического сада. 2016. С. 152-153. РИНЦ

10. Romadanova N., Kushnarenko S., Karasholakova L. Development of a common PVS2 vitrification method for cryopreservation of several fruit and vegetable crops//In Vitro Cellular & Developmental Biology – Plant. – 2017. Volume 53, Issue 4, — P. 382–393. DOI 10.1007/s11627-017-9849-y. Scopus 51 процентиль, WoSQ2

11. Ромаданова Н. В., Серадж Н. А., Нурманов М. М., Карашолакова Л. Н. Введение в культуру in vitro дикорастущей яблони Malus sieversii / поиски, результаты – исследование, результаты. – 2017. № 3 (75). — С. 103-110. КОКСОН

12. Romadanova N.V., Mishustina S.A., Karasholakova L.N., Kushnarenko S.V. Optimization of barberry micropropagation and cryopreservation // 2nd International Conference Plant Cells In Vitro: Fundamentals & Applications, Vienna,Austria, June 26-27, 2017. — P. 23.

13. Romadanova N.V., Machmutova I.A.,Karasholakova L.N., Kushnarenko S.V. Optimization of barberry micropropagation/ / Научный журнал «Eurasian Journal of Applied Biotechnology», 2017, № 2. DOI: 10.11134 / btp.2.2017.7 КОКСОН, ВИНИТИ