Processing of food (bio-waste) waste with the help of larvae of the Black Soldier Fly (Hermetia illucens) and production of feed protein

Science

    Project title:“Processing of food (bio-waste) waste with the help of larvae of the Black Soldier Fly  (Hermetia illucens) and production of feed protein” (project duration 12 months, 01.01.-31.12.-2022)

    Urban waste management is considered one of the most urgent and serious environmental problems. The severity of this problem will increase in the future, given the rapid growth of the urban population. Due to the growing public pressure and environmental problems, it is necessary to develop more sustainable methods of handling household waste. This project is largely based on the experience of operating a plant for processing one ton of waste per day. The waste processed at the enterprise is mainly fruit and vegetable waste from the wholesale market. It should be understood that scaling to a larger object may require some adaptation or equipment configuration. However, we believe that the standard procedures described are applicable for a wide range of production scaling. The processing of organic waste (bio-waste) is still quite limited, especially in Kazakhstan, although it is by far the largest part of all municipal waste generated. This project examines urban organic municipal waste from households, commercial enterprises and institutions. It describes a fairly new, but at the same time practically proven approach to the transformation of biological waste by insect larvae on the example of the black lion fly (CHL), Hermetia illucens; an approach that has attracted a lot of attention in the last decade. Its popularity is associated with the promising possibilities of using collected CHL larvae as a source of feed protein for animals, which provides a valuable alternative to traditional feed.

Key characteristics make the technology an attractive option for processing bio-waste from the point of view of recycling tasks by food and agricultural enterprises. Let ‘s list them:

  • Waste biomass turns into larvae and vermicompost. Larvae consist of 35% protein and 30% crude fat. This insect protein is of high quality and is an important feed for poultry and fish farmers. Testing of feeds by foreign specialists has confirmed that this is a suitable alternative to the selected meal.
  • It has been shown that when waste is processed by Black Lion larvae, disease-carrying bacteria such as salmonella are inactivated in the waste. This means that the risk of disease transmission between animals and between animals and humans is reduced when using this technology at the farm level or when processing animal waste in general (for example, chicken manure or slaughterhouse waste).
  • Such treatment of organic waste can reduce the number of open landfills, which is still a sad reality.
  • Waste processed by larvae is called vermicompost. This compost-like substance contains nutrients and organic substances and, when used in agriculture, helps to reduce soil depletion.
  • Demonstrated a high degree of conversion of waste into biomass, up to 25% in terms of raw mass, which is a satisfactory solution from the point of view of technology and business.
  • There is no need for complex high-tech solutions to operate such an object. Thus, it is suitable for regions that rely mainly on simple technologies and unskilled labor.

The technological solution consists in feeding segregated bio-waste to larvae of the Black Lion Fly (CHL) grown in a nursery. Larvae grow on waste and reduce the mass of waste. At the end of the process, the larvae are collected and processed into suitable food for animals, birds and fish. The remaining waste will also be recycled and potentially used as a soil fertilizer.

  Project goal: To study the full life cycle of the Hermetia illucens Black Lion fly, to collect statistical data on waste processing by the larvae of the Black Lion fly, to determine the potential and design capacity of this technology.

 Expected results:

  1. processing of organic waste – 150 tons
  2. growing a live larva – 50 tons
  3. production of dry larva – 10 tons
  4. production of protein-lipid concentrate – 2 tons
  5. production of fat – 2 tons
  6. production of vermicompost – 50 tons
  7. A certificate of copyright registration will be received.
  8. It is also planned to publish the work in domestic and foreign scientific journals.

 Project Manager:

 Aigerim Kambarova, Master of Natural Sciences -. Since 2019, she has worked as the head of the Bioresources Laboratory, the Research Institute of Biotechnology Problems of I.Zhansugurov Zhetysu University. He has more than 25 publications, including 1 publication in the Web of Science and Scopus databases, COXON -5, international conferences – 4, books – 1, patent – 1. ORCID 0000-0003-1317-5080 Link to profile https://orcid.org/my-orcid?orcid=0000-0003-1317-5080

 Researchteammembers:

 Karipbaeva Rasima Kurmanalievna, 1st year doctoral student OP 8D01503- biology. more than 20 scientific papers, including 1 article in the Web of Science and Scopus databases, 3- Coxon, international conferences -, books-1, patent-1. ResearcherID ABF-7082-2021, Scopus ID 57219090218,ORCID ID 0000-0001-8187-1614

Zhakupzhanova Madina Farhadovna – has published more than 10 scientific papers; 1 of them is News of NAS RK, 6 international conferences, -1 patent.

Scientific internships and advanced training

  1. Aigerim Kambarova 21.11.2016-26.11.2016 – research internship at the Institute of General Genetics and Cytology, Almaty, Kazakhstan. Practical training on microclonal reproduction of woody plants. Mastering the methodology: formation of the composition and preparation of nutrient media for explant cultivation; microcloning microclones in vitro; cultivation of regenerants of wood crops in container culture.
  2. Aigerim Kambarova 11.09.2017-22.09.2017 University of Zilina, Slovakia, Institute of High Mountain Biology Postmastership Training.
  3. Aigerim Kambarova 28.06.2018 – 06.07.2018 – research internship in biological plant Protection, Department of Plant Protection, Faculty of Agriculture, Kyrgyz-Turkish University “Manas”, Bishkek, Kyrgyzstan. The purpose of the research internship: modern methods for the rapid identification of microorganisms and pests that cause diseases in plants; diagnostics of plant diseases and pests; scientific foundations for the creation of disease-resistant and pest-resistant varieties of agricultural plants.
  4. Kambarova Aigerim 11.03.2019-16.03.2019 research internship on the determination of reserves (biological, operational) useful and natural learning conditions. Institute of Chemistry and Biotechnology of Bread KR. Bishkek, Kyrgyzstan.
  5. Kambarova Aigerim 02.10.2019-12.10.2019 educational and research internship on the technology of deepening and expanding lecture, essential and useful plants in the usnov culture at the Institute of Chemistry and Phytotechnology Tan KR, Bishkek, Kyrgyzstan.
  6. Kambarova Aigerim 11.03.2019-16.03.2019 educational and research internship on Obtaining new highly effective growth stimulants and biologically active compounds; at the Institute of Chemistry and Phytotechnology bread from the Kyrgyz Republic, Bishkek, Kyrgyzstan.
  7. Zhakupzhanova M.F. 11.03.2019-16.03.2019-Technology of cultivation and breeding of lecture, essential and useful plants in crop improvements, Institute of Chemistry and Phytotechnologies of the Kyrgyz Republic, Bishkek, Kyrgyzstan.

    List of publications and patents of the Project executors for the period from 2015 to 2020.

  1. Kambarova Ғылыми – техникалық прогресс дамуының Қазақстан экологиясына әсері // Materials of the XIV annual International Scientific and Practical conference of students, undergraduates, doctoral students, postgraduates and applicants “Socio-economic development of the society of the future: trends, growth points, effective solutions” – 2015. – С. 537-540.
  2. Kambarova , Zhakupzhanova M.F.Применение зеленого черенкования для размножения плодовых и декоративных растений // Materials of the Republican scientific and practical conference “Youth and science in the modern world. – 2015. – pp. 62-67.
  3. Bakhtaulova A.S., Kambarova , Zhakupzhanova M.F Применение зеленого черенкования для размножения плодовых и декоративных растений // Materials of the XXIX student international correspondence scientific and practical conference “Youth Scientific Forum: Natural and medical Sciences”. – 2015. № 10 (28). – P. 10-18. **The collection is included in the scientific and metric database of the RSCI
  4. Kambarova , Zhakupzhanova M.F. Жасыл қалемшелеу арқылы сәндік және цитрусты бөлме өсімдіктерін көбейту // Materials of the Republican scientific and practical conference” youth and science in the new world”. – 2016. – Pp. 300-305.
  5. Bakhtaulova A.S.,Kambarova , Zhakupzhanova M.F.. Влияние регуляторов роста на корнеобразовательную способность зеленых черенков декоративных растений // International scientific journal “The Way of Science”. – 2017. № 2 (36). – Pp. 32-36. *Impact factor of the journal “Path of Science” – 0.543 (Global Impact Factor, Australia), – 0.350 (Open Academic Journals Index, Russia
  6. Bakhtaulova A.S., Shakenova E.A., Kambarova A., Zhakupzhanova M. Микроклональное размножение яблони Сиверса (Malus sieversii) // Bulletin of the State University named after Shakarima. – 2018. № 3 (83).
  7. Zhakupzhanova M.F., Kanaev A.T., Kambarova A. Роль регуляторов роста на укоренение зеленых черенков комнатных растений в климатической камере XVI All-Russian school with international participation – seminar on structural macrokinetics for young scientists named after academician A.G.Merzhanov. Chernogolovka. October 10-12, 2018 / Collection of materials. – Chernogolovka, ISMAN. – 2018. P – 124-126. ISBN 978-5-9907575-3-0, DI: 10.24411/9999-004A-2018-10064
  8. Ashimkhan Kanayev, Kuat Baymyrzayev, Aigerim Kambarova, Madina Zhakupzhanova, Rauan Satymbekov,  DEVELOPMENT OF AN EFFECTIVE METHOD OF PRE-SEED TREATMENT IN SEEDS OF SUGAR BEET (PAPER publishing) //The Review Committee came to a conclusion that the proposed theme/s is/are in the scope of the 19th International Multidisciplinary Scientific Geo Conference& EXPO SGEM2019 and will be of a great interest to the SGEM2019 participants. SCOPUS
  9. Kanaev A.T., Baymyrzaev K.M., Kambarova A., Zhakupzhanova M. Heterocarpia in relation to sugar beet seed protectants and their productivity // Trends in the development of modern science and education: traditions, experience, innovations: Collection of scientific articles based on the materials of the All-Russian Scientific and Practical Conference (with international participation), Republic of Bashkortostan, Sibai / 2018. P – 13-14.
  10. Kanaev A.T., Kambarova A., Zhakupzhanova M., The study of the radial parenchyma and the root-forming ability of the common lemon (Citrus limon (L.) Burm.) with green cuttings / International seminar on the study of biodiversity at the Varzob mining and Botanical station “Kondara”+ publication / 2019.
  11. A. Kambarova, M.F. Zhakupzhanova Селекция и сорторазведение садовых культур 2019. Vol. 6. No. 1 Materials of the international scientific and practical conference (with international participation), “Selection is the basis for the development of intensive horticulture”, FGBNU VNIISPK, Orel. ISSN 2500-0454. Pages: 14-17.
  12. A. Kambarova, M.F. Zhakupzhanova Определение солеустойчивости риса посевного (ORYZA SATIVA) в условиях орошаемого земледелия алматинской области // “Bulletin of Karaganda University”, series “Biology, Medicine, Geography” No. 3, 2019.
  13. Bakhtaulova A.S., Kambarova A., Zhakupzhanova M.F Морфоанатомические особенности строения стебля лимона обыкновенного (Сitrus limon (l.) Burm.) SELECTION AND VARIETY BREEDING OF GARDEN CROPS 2019. Vol. 6. No. 1 “SELECTION is the basis for the development of intensive gardening”, FGBNU VNIISPK, Orel. ISSN 2500-0454. Pages: 14-17.
  14. Kambarova A. Kanaev A.T. Satymbekov R. Karipbaeva R.K., S. Turlikotzha.N. Sartbayeva Zh.B. Разработать Приемы управления продукционным процессом  Озимой пшеницы для технологий различного уровня интенсификации в системе точного земледелия \ “SUSTAINABLE DEVELOPMENT OF TERRITORIES:THEORY AND PRACTICE”Materials of the X All-Russian Scientific and Practical Conference (Sibai, November 14-16, 2019), pp. 124-126.
  15. Kambarova A., Kanaev A.T., Satymbekov R., Karipbaeva R.K.  Лучeвая пapeнхима и кopнeoбpaзoвaтeльные cпocoбнocти  лимона обыкновенного (citrus limon (l.) Burm.) //”SUSTAINABLE DEVELOPMENT OF TERRITORIES:THEORY AND PRACTICE”Materials of the X All-Russian Scientific and Practical Conference (Sibai, November 14-16, 2019), pp. 126-128.
  16. Kambarova A., Kanaev A.T., Satymbekov R., Karipbaeva R.K. Размножение декоративных растений путем зеленого черенкования с применением регуляторов роста // “SUSTAINABLE DEVELOPMENT OF TERRITORIES:THEORY AND PRACTICE”Materials of the X All-Russian Scientific and Practical Conference (Sibai, November 14-16, 2019), pp. 129-131.
  17. A.T. Kanaev, A. Kambarova, R.Satymbekov , R.K. Karipbaeva Применение способа микроклонального размножения смородины мейера в Казахстане // “SUSTAINABLE DEVELOPMENT OF TERRITORIES:THEORY AND PRACTICE”Materials of the X All-Russian Scientific and Practical Conference (Sibai, November 14-16, 2019), pp. 131-133.
  18. Kambarova A., Kanaev A.T., Karipbaeva R.K., Satymbekov R.K.. Целесообразность применения методов in vitro для размножения смородины мейера в казахстане // IV International Scientific and Practical Conference “SCIENCE AND EDUCATION IN THE MODERN WORLD: CHALLENGES OF THE XXI century” NUR-SULTAN – 2019, – pp. 76-79.
  19. A patent for a utility model. No.105504 “Method of propagation of the Sievers apple tree (Malus sieversii) with green cuttings. Kambarova Aigerim and Bakhtaulova A.S., Kanagatov Zh.Zh., Topaev O.S., Oksikbaev B.K., Zhakupzhanova M.F. authors of the utility model.

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