Name of the topic of the project. Study and conservation of endangered species of Oxytropis by biotechnology methods

Goal 1 – Phytochemical study of poorly studied species of acorn by gas chromatography with flame ionization detector (GC/FID) and mass spectrometry (GC/MS);

Goal 2 is the conservation of endangered species of holly, including rare and endemic ones, by biotechnology methods (tissue culture method, cryopreservation).

Objectives of the project:

1. Search for populations of rare and endemic species of Oxytropis;

2. Collection of plant material for in vitro culture, creation of a seed collection and phytochemical analysis;

3. Analysis of the quantitative and qualitative composition of plant parts using gas chromatography with a flame-ionization detector (GC/FID) and mass spectrometry (GC/MS) (at least 2 species of Oxytropis);

4. Introduction into in vitro culture and micropropagation to create a collection of in vitro aseptic plants (at least 3 species of Oxytropis);

5. Creation of an in vitro collection of aseptic plants (at least 3 species of Oxytropis);

6. Creation of a seed collection at temperatures of +4°C, -20°C, as well as a cryocollection in liquid nitrogen at -196°C (at least 3 species of Oxytropis).

The idea of the project:

The idea of the project is a comprehensive study of unexplored Kazakh Oxytropis species using plant biotechnology methods (tissue culture method, cryopreservation) and gas chromatography (GC/FID, GC/MS).

The project fills existing gaps and provides valuable knowledge phytochemistry and conservation of Kazakh Oxytropis, and contributes to effective management of plant resources. The obtained material and data will serve as the basis for next projects on genotyping and biological activity of Oxytropis.

Thus, the idea of the project fits into the global trend of preserving biodiversity, and is also new, relevant and timely for Kazakhstan and the object of study.

Expected results:

For the first time there will be:

1. Analysis of quantitative and qualitative composition of plant parts was carried out using gas chromatography with flame ionization detector (GC/FID) and mass spectrometry (GC/MS) (at least 2 Oxytropis species).

2. Introduced into in vitro culture and micropropagated to create a collection of aseptic plants (at least 3 Oxytropis species).

3. The collection of in vitro aseptic plants of Kazakhstani Oxytropis species was created (at least 3 species).

4. The seed collection of at temperatures of +4°C, -20°C, as well as a cryogenic collection of seeds in liquid nitrogen at a temperature of -196°C was created (at least 3 Oxytropis species).

The obtained information about the component composition of Oxytropis can be used to create new broad-spectrum drugs.

The obtained information about the component composition of Oxytropis can be used to create new broad-spectrum drugs.

The created collections (seed collection, cryocollection, in vitro aseptic plant collection) can serve as material for restoring rare, endemic Oxytropis species. Also, this plant material can be used for exchange of genetic material between domestic and foreign research organizations in order to replenish their field and in vitro collections, as well as seed banks.

Will be prepared and published:

– at least 2 articles in journals indexed in SCIE and included in 1-2-3 quartile (Q1, Q2, Q3) by impact factor in Web of Science and/or in journal with a percentile of at least 50 in Scopus, at least 2 articles in publications recommended by the Committee for Control in Field of Science and Higher Education from lists 1 and 2;

– or at least 1 article in journal indexed in SCIE and included in 1st quartile (Q1) by impact factor in Web of Science and/or in journal with a percentile of at least 80 in Scopus, at least 1 articles in publications recommended by the Committee for Control in Field of Science and Higher Education from lists 1 and 2;